Complete Human Skin Grown From Stem Cells

Complete human skin epidermis has been successfully grown skin-derived stem cells on laboratory mice. This advance could lead to production of one's own skin (an autologous transplant) without the immediate need to harvest the skin from other areas of the body.


(Complex structure of human epidermis)

Background
Cell therapy for large burns is dependent upon autologous epidermis reconstructed in vitro. However, the effectiveness of current procedures is limited by the delay needed to culture the patient's own keratinocytes. To assess whether the keratinocyte progeny of human embryonic stem cells (hESCs) could be used to form a temporary skin substitute for use in patients awaiting autologous grafts, we investigated the cells' capability of constructing a pluristratified epidermis.

Methods
hESCs from lines H9 and SA01 were seeded at least in triplicate on fibroblast feeder cells for 40 days in a medium supplemented with bone morphogenetic protein 4 and ascorbic acid. Molecular characterisation of cell differentiation was done throughout the process by quantitative PCR, fluorescence-activated cell sorting, and immunocytochemical techniques. Keratinocyte molecular differentiation and functional capacity to construct a human epidermis were assessed in vitro and in vivo.

Findings
From hESCs, we generated a homogeneous population of cells that showed phenotypic characteristics of basal keratinocytes. Expression levels of genes encoding keratin 14, keratin 5, integrin α6, integrin β4, collagen VII, and laminin 5 in these cells were similar to those in basal keratinocytes. After seeding on an artificial matrix, keratinocytes derived from hESCs (K-hESCs) formed a pluristratified epidermis. Keratin-14 immunostaining was seen in the basal compartment, with keratin 10 present in layers overlying the basal layer. Involucrin and filaggrin, late markers of epidermal differentiation, were detected in the uppermost layers only. 12 weeks after grafting onto five immunodeficient mice, epidermis derived from K-hESCs had a structure consistent with that of mature human skin. Human involucrin was appropriately located in spinous and granular layers and few Ki67-positive cells were detected in the basal layer.

Interpretation
hESCs can be differentiated into basal keratinocytes that are fully functional—ie, able to construct a pluristratified epidermis. This resource could be developed to provide temporary skin substitutes for patients awaiting autologous grafts.

Artificial skin is a science fiction staple; see the surrogate skin from Robert Heinlein's 1951 novel The Puppet Masters and art-derm from Philip K. Dick's 1961 novel Dr. Futurity and the more functional read-out skin from John Varley's 1992 novel Steel Beach.

Looking for more real-life artificial skin alternatives? I've got you covered:

From The Lancet via medGadget.

Scroll down for more stories in the same category. (Story submitted 12/3/2009)

Follow this kind of news @Technovelgy.

| Email | RSS | Blog It | Stumble | del.icio.us | Digg | Reddit |

Would you like to contribute a story tip? It's easy:
Get the URL of the story, and the related sf author, and add it here.

Comment/Join discussion ( 0 )

Related News Stories - (" Medical ")

SpinCare Electrospins And Shoots Wound Dressing
'Over her lacerated right shoulder he sprayed art-derm...' - Philip K. Dick, 1960

PEDOT Polymer Could Enhance Brain-Machine Interfaces
'the hair-fine wire going deep into Owen's brain, down into the pleasure center.' - Larry Niven, 1969.

BioVYZR Is Ready, Anti-Covid19 PAPR Lovers
'Some clad in the insulated space-suits, with their transparent glassite helmets.' - Edmond Hamilton, 1931.

Healight Ultraviolet Endotracheal Device Has Covid-19 Treatment Potential
'He applied the tip of the instrument to the interior of the wound...' - Cogswell, 1976.

 

Google
  Web TechNovelgy.com   

Technovelgy (that's tech-novel-gee!) is devoted to the creative science inventions and ideas of sf authors. Look for the Invention Category that interests you, the Glossary, the Invention Timeline, or see what's New.

 

 

 

 

 

Current News

Listen Up, Coppertop - Wearable Device Turns You Into A Battery
'It's our way or the highway.'

Solar Powered Robot Cleans Up Solar Panels For Free
'... with large padded feet, who were apparently polishing their way the whole length of Rama's six artificial suns.'

Spot Arm From Boston Dynamics Picks Up Like Heinlein's Hired Girl Robot
'Anything larger than a BB shot it picked up and placed in a tray on its upper surface...'

Electric Vehicle Prices Will Drop To $2,890
'the human seats took up two-thirds of the room in each'

TIGER Ultimate Mobility Vehicle From Hyundai
'He admired the fast-plodding, articulated legs...'

Drones Communicate With Each Other Using Quantum Encryption
'the curious absent look of a robot talking on the TBR circuits...'

Space Construction Tools For Large Structures By OAC
'Mass-produced in the orbiting factories...'

SpinCare Electrospins And Shoots Wound Dressing
'Over her lacerated right shoulder he sprayed art-derm...'

Tesla Model S And X Moves Toward KITT Knight Rider Styling
Now, that was a Pontiac Trans Am with some real style.

Sophia Robots By The Thousands
'There is a built-in microphone that hears everything you say, and an electric brain that selects a suitable answer.'

Scaly-Foot Snail Works With Iron
'Micro-Scale suit tiles fabricated by genetically engineered metal affinity bacteria...'

Updated Piaggio Gita Personal Robot Porter
'Carry his bag... and follow him faithfully...'

Las Vegas Tunnels To Have Autonomous Teslas
'...just a steady velvety whirr as the taxi sped along.'

TCL CSOT 17-Inch Printed OLED Scrolling Display
'..a wide sheet of clear material suddenly flared with light and swirling colour.'

Reachy Humanoid VR Teleoperation App
"I went to the control room where the three other men were manipulating their mechanical men...'

Unitree A1 Robot ala Black Mirror and Snow Crash
'The legs are long, curled way up to deliver power...'

More SF in the News Stories

More Beyond Technovelgy science news stories

Home | Glossary | Invention Timeline | Category | New | Contact Us | FAQ | Advertise |
Technovelgy.com - where science meets fiction™

Copyright© Technovelgy LLC; all rights reserved.